Search results

Quantitative PCR as a predictor of aligned ancient DNA read counts following targeted enrichment

McMaster Ancient DNA Centre research on the use of quantitative PCR to predict hybridization capture success.

Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

Here we report a systematic comparison of formalin-fixed paraffin-embedded tissue kits for RNA and DNA extraction. Overall, TrimGen's WaxFree and ...

Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

We report the sequencing of a remarkably well-preserved Siberian woolly mammoth. Using a metagenomics approach we sequenced about 28 million reads of which ...

Isolation of nucleic acids and cultures from fossil ice and permafrost

A review of recent claims about viable cells and microbial nucleic acids obtained from ice and permafrost hundreds to millions of years old. We also outline a ...

A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts

PCR inhibition remains a consistent problem for PCR amplification, particular in situations when the target DNA is in low-abundance or heavily degraded. Here ...

Time-Dependency of Molecular Rates in Ancient DNA Datasets, a Sampling Artifact?

Here we examine how a variety of factors (including calibration methods, sequence length, etc) affect divergence estimates in BEAST.

Long-Term Survival of Ancient DNA in Egypt: Response to Zink and Nerlich (2003)

A response to the arguments about the survival of ancient DNA in Egypt and the likelihood of retrial of ancient DNA from Egyptian remains.

Congratulations Katherine Eaton, MacData Fellow

Congratulations to Katherine Eaton on being named a MacData Fellow. Her proposed research focuses on mining vast international repositories of DNA sequencing ...

Congratulations Tyler Murchie

The Mac aDNA Centre would like to congratulate Tyler Murchie on the successful defence and submission of his PhD Dissertation.

The Recovery, Interpretation and Use of Ancient Pathogen Genomes

A review of the history of ancient DNA in pathogen and pandemic research and how we interpret the spread of diseases in the past.