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The top 10 list: criteria of authenticity for DNA from ancient and forensic samples

A summary of 10 criteria to ensure replication and authentication of ancient DNA recovered from ancient and forensic samples.

Ancient DNA

A review of the field of ancient DNA, focusing on the technical pitfalls and stringency needed to ensure reproduciblity and authenticity of results.

A draft genome of Yersinia pestis from victims of the Black Death

This paper reports the first complete genome of the causitive agent behind the Black Death, Yersinia pestis. Notably it also shows that modern Yersinia pestis ...

Tarsus determination in Drosophila melanogaster

Here we report our work looking into tarsus determination in Drosophila as it relates to ANTP cell autonomous or noncell autonomous determination.

Ancient human genomics: the methodology behind reconstructing evolutionary pathways

A review of the processes undertaken in Ancient DNA studies - from sample to data analysis.

Quantitative PCR as a predictor of aligned ancient DNA read counts following targeted enrichment

McMaster Ancient DNA Centre research on the use of quantitative PCR to predict hybridization capture success.

Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

Here we report a systematic comparison of formalin-fixed paraffin-embedded tissue kits for RNA and DNA extraction. Overall, TrimGen's WaxFree and ...

Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA

We report the sequencing of a remarkably well-preserved Siberian woolly mammoth. Using a metagenomics approach we sequenced about 28 million reads of which ...

Isolation of nucleic acids and cultures from fossil ice and permafrost

A review of recent claims about viable cells and microbial nucleic acids obtained from ice and permafrost hundreds to millions of years old. We also outline a ...

A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts

PCR inhibition remains a consistent problem for PCR amplification, particular in situations when the target DNA is in low-abundance or heavily degraded. Here ...